Bioinformatics tools used in the study of phenoloxidases enzymes of the genus Pleurotus

Abstract

The bioinformatic analysis allows relating, predicting and analyzing a set of proteins of interest that share conserved areas in common by using a computer (in silico). This bioinformatic analysis is made from the sequence of databases, domains and key regions that make up the catalytic function of a protein. The amino acid sequence provides a prediction corresponding to the conserved regions. Subsequently, the amino acid sequences are coupled in an orderly manner by their function and structure, which generate spatial projections of tertiary structures that allow the recognition of key sequences for coordinated mutations. Bioinformatics in phenoloxidase enzymes such as laccase, manganese peroxidase and decolorizing peroxidase allows observing protein structures, alpha and beta chains as well as the ions responsible for catalysis. In silico techniques play an important role in different areas such as medicine, biotechnology and genomics for the benefit of human health. The DNA sequencing technique identifies organisms by means of amino acid sequences taken from database to make template proteins, sequence alignment, homology modeling and the validation or identification of organisms.