Design of bioprocesses and bioproducts in plant cell engineering

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Hebert Jair Barrales-Cureño
César Reyes-Reyes
Maximino Díaz-Bautista
Alejandro Pérez-Rosales
Arturo Castañeda-Mendoza
Jesús Eduardo Zaragoza-Ruíz
Petra Andrade-Hoyos
Alfonso Luna-Cruz
Jordi Orlando Osuna-González
Luis Germán López-Valdez
Salvador Chávez-Salinas

Abstract

Currently, the effective application of bioprocesses in plant and cell systems has proved to be successful in the production of secondary metabolites. The in vitro plant cell culture is an alternative used in their biotechnological production. However, synthesis is a complex factor observed at laboratory tests. The wide range of metabolites that are synthesized by plant cells include drugs, flavors, scents, cosmetics, natural pigments and pesticides. Rheology, aggregation, light, pH, and temperature, are factors considered in the design of bioprocesses and bioproducts, including agitation, blending, aeration, oxygen concentrations and hydrodynamic shear force. Other important elements are the mode of cultivation and the type of bioreactor used. This work presents the advantages and disadvantages observed in the use of bioreactors for plant cell culture. Similarly, it considers the optimal development of secondary metabolites for future investigations when the biosynthesis occurs. Other relevant aspects are also included in this work, such as biosecurity and economy.

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