Establishment in vitro shoots and callus Dahlia sp. for later use to obtained inulin

Abstract

In order to have an efficient protocol and to be able to produce inulin in the future, experimental conditions were established to obtain artificial cultures of Dahlia sp. plant lines A1, A2, P1, P2 and ENN to obtain shoots, calli, cell and root suspension cultures with different combinations of plant growth regulators and environmental conditions. The explants used were axillary buds. With these, shoots and whole plants were obtained in all lines. The P1 line was the fastest, needed 7 days of incubation to respond to MS medium without plant growth regulators. Calli were obtained with stem explants after 10 days with 0.5 mg/L benzylaminopurine (BAP) and 1 mg / L indoleacetic acid (IAA). Calli suspension cultures were achieved with stirring at 88 rpm and the same plant growth regulators combinations that were obtained for calli formation. Tuberization induction and root formation were with 5 mg/L BAP, 0.5 mg/L IAA, 80 g/L sucrose and 0.2 mg/L BAP plus 60 g/L sucrose, respectively. The response time and the quality of buds, calli and roots obtained from Dalhia sp. showed favorable experimental conditions to initiate the experiments for the stimulation of inulin production.