Isolation and identification of microorganisms with capacity to produce indole-acetic acid in onion crops of the state of Zacatecas

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Hádrian González-Torres
Arturo Alejandro Rodríguez-Moreno
María Laura Eugenia Romero-Jaramillo
Verónica Segovia-Tagle
Francisco Alejandro Paredes-Sánchez
Karol Karla García-Aguirre
Elsa Verónica Herrera Mayorga
Leticia Bravo-Luna

Abstract

Growth promoting bacteria have the ability to function as biofertilizers that provide the plant nutrients, as well as the production of phytohormones such as indole-acetic acid (IAA). The evaluated strains were isolated from onion crops soil of the city of Fresnillo, Zacatecas, Mexico. Six strains positive for the production of indole-acetic acid were obtained. All six strains were identified by DNA extraction and subsequent bidirectional sequencing of the 16S gene. The strain identified as Pseudomonas cedrina was the largest producer of indole-acetic acid with a value of 174 ± 0.06 μg / mL; the bacteria identifided have potential to be used in the formulation of biofertilizers.

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