Molecular characterization of pathogenic bacteria by pulsed field-gel electrophoresis (PFGE)

Abstract

The growing need for surveillance of re-emerging infectious diseases necessitates the application of molecular characterization methods such as Pulsed-Field Gel Electrophoresis -PFGE. In the first collection, analysis macrorestriction S. aureus reveals a medium frequency (0.54) of pulsetype II, compared to the second collection where increased genetic heterogeneity exists. S. enterica presented some identical pulsotypes and other pulsotypes presented a genetic diversity (DI = 0.95), which suggests that there is epidemiological unrelated, with pulsetypes to particles serotypes. In addition, this study will help us create our database according to the PulseNet International Center for Disease Control and Prevention (CDC, USA).