Developing a culture medium suitable for the production of lactic acid bacteria from agro-industrial waste

Abstract

The importance of lactic acid bacteria (LAB) in the world, whether in food, necessary to optimize methods and develop a suitable environment for the growth of these microorganisms, using different substrates from agroindustrial wastes for development. Molasses, whey, mead and apple juice from cider production as a substrate of BAL (Pediococcus, Lactobacillus, Lactococcus) was used. Conditions and variables influencing microbial growth were evaluated. Four means of various growth for each of the aforementioned substrates from a MRS broth medium based modifying its carbon source were prepared. were inoculated with 10 strains BAL a media control (substrate only inoculated) and supplemented medium (substrate with the addition of mineral salts) at different concentrations of molasses, whey, mead and juice sterile cider adjusting its pH to 6.4 ± 0.2. Two variables were handled without agitation. Biomass production was determined using direct counting on MRS agar plate. The interpretation of results was performed from the count of CFU per mL according to the microbiological count. The optimal environment for the growth and biomass production of BAL medium mead was 12.3 °Brix supplemented with mineral salts, in an agitated. Mead was concluded that can be used as substrate for the growth of LAB.