Growth of soil lipolitic microorganisms in different culture media with vegetal oil

Abstract

Specific culture media are used for lipolytic fungi and bacteria growth. It is possible that more complex culture media, used for plant tissue culture, allows a higher expression of fungi and bacteria growth. The aim was to evaluate the effect of the addition of vegetable oil in different culture media for micropropagation, on growth of fungi and bacteria from soil samples. Ten soil samples from different sites in Chiapas, two types of culture media (MS with 1.48 mg/L TDZ and 1 mg/L AIB; Yasuda with 1.25 mg/L KIN and 1 mg/L AIB) and the presence or absence of olive oil (1%) in the culture media were evaluated. Expression of fungi and bacteria was significantly affected by at least two evaluated factors. The factor soil sample led fungal expression percentages from 47.5 to 100%, while in bacteria it was from 95 to 100%. The presence of olive oil favored fungal expression (94.5%). Both culture media allowed the growth of microorganisms. The vegetable oil factor promoted 94.5% of fungi expression and 99.5% of bacteria. More diversity was found in fungi expression compared to bacteria. Differences in microorganisms growth were mainly due to the soil sample.