Leaf-derived organogenesis from lisianthus (Eustoma grandiflorum) in response to different plant growth regulators

Abstract

Lisianthus´ characteristics make it a cut flower of great interest, so the use of micropropagation techniques favors a rapid and massive multiplication, with plants free of pathogens. The organogenesis, resulted of use regulators of growth of different action mode (auxin, cytokinin and gibberellin), included the formation of shoots and roots that it was generated in some cases directly and in other from callus, employing the foliar segments as explant. The addition of low concentrations of cytokinin and gibberellin (1.0 mg/L benzyladenine (BA) + 0.5 mg/L gibberellic acid (GA3)) to the culture medium were necessary to generate the highest number of shoots (14.16 shoots per explant).