Food interest Agave salmiana’s fructans obtention using plant tissue culture techniques

Abstract

The objective of this work was to establish the disinfection protocol for Agave salmiana in vitro culture, to be able to submit the explants to different vegetal growing regulation concentrations for callus induction and the later obtaining of fructans. The explants were incubated in Murashige & Skoog media, different types of 2,4-D and BAP combinations were used and it was solidified with phytagel. The explants submitted to low lightning conditions and low concentrations of vegetal growing regulators (VGR) were oxidized faster than the ones that were submitted to darkness and higher concentrations of VGR. In closer concentrations between 2,4-D and BAP, traces of callus were observed and root traces in the ones with higher concentration of 2,4-D. The explants used as control behaved differently, in Agave tequilana the explant increased in size compared to Agave salmiana without the formation of viable callus, and for the asparagus, callus was already observed. Histological study was made of the explants of Agave salmiana, the explant of immature leaves contained few core cells, important for the formation of the desired callus.